• Korea University Department of Chemical & Biological Engineering
  New Engineering Hall, #713, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea


• Tel:  02-3290-4850
• FAX: 82-2-926-6102
• E-mail: jbkim3@korea.ac.kr

EXPERIENCES

EDUCATION

HONORS

국제학술지

• 1. Jungbae Kim and Jonathan S. Dordick, “Pressure Affects Enzyme Function in Organic Media”, Biotechnol. Bioeng., 42, 772-776 (1993).


• 2. Keungarp Ryu, Jungbae Kim and Jonathan S. Dordick, “Catalytic Properties and Potential of an Extracellular Protease from an Extreme Halophile”, Enzyme Microb.Technol., 16, 266-275 (1994).


• 3. Jungbae Kim and Jonathan S. Dordick, “Unusual Salt and Solvent Dependence of a Protease from an Extreme Halophile”, Biotechnol. Bioeng., 55, 471-479 (1997).


• 4. Jungbae Kim, Xiaoqiu Wua, Michelle R. Hermana and Jonathan S. Dordick, “Enzymatically-Generated Polyphenols as Array-Based Metal-Ion Sensors”, Anal. Chim. Acta, 370, 251-258 (1998).


• 5. Jungbae Kim, David H. Altreuter, Douglas S. Clark, and Jonathan S. Dordick, “Rapid Synthesis of Fatty Acid Esters for Use as Potential Food Flavors”, J. Am. Oil Chem. Soc., 75, 1109-1113 (1998).


• 6. Jungbae Kim, Douglas S. Clark and Jonathan S. Dordick, “Intrinsic Effects of Solvent Polarity on Enzymic Activation Energies”, Biotechnol. Bioeng., 67, 112-116 (2000).


• 7. Xiaoqiu Wua, Jungbae Kim and Jonathan S. Dordick, “Enzymatically and Combinatorially Generated Array-Based Polyphenol Metal Ion Sensor”, Biotechnol. Progress, 16, 513-516 (2000).


• 8. Jungbae Kim, Timothy J. Kosto II, Joseph C. Manimala, E. Bruce Nauman and Jonathan S. Dordick, “Preparation of Enzyme-In-Polymer Composites with High Activity and Stability”, AIChE Journal, 47, 240-244 (2001).


• 9. Jungbae Kim, Ray Delio and Jonathan S. Dordick, “Protease-Containing Silicates as Active Antifouling Materials”, Biotechnol. Progress, 18, 551-555 (2002).


• 10. Jungbae Kim and Jay W. Grate, “Single-Enzyme Nanoparticles Armored by a Nanometer-scale Organic/Inorganic Network”, Nano Lett., 3, 1219-1222 (2003).


• 11. Thurston E. Herricks, Sae-Hoon Kim, Jungbae Kim, Dan Li, Ja Hun Kwak, Jay W. Grate, Seong H. Kim and Younan Xia, “Direct Fabrication of Enzyme-Carrying Polymer Nanofibers by Electrospinning”, J. Mater. Chem., 15, 3241-3245 (2005).


• 12. Jinwoo Lee, Jungbae Kim, Jaeyun Kim, Hongfei Jia, Moon Il Kim, Ja Hun Kwak, Sunmi Jin, Alice Dohnalkova, Hyun Gyu Park, Ho Nam Chang, Ping Wang, Jay W. Grate and Taeghwan Hyeon, “Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates”, Small, 1, 744-753 (2005).


• 13. Jinwoo Lee, Dohoon Lee, Eunkeu Oh, Jaeyun Kim, Young-Pil Kim, Sunmi Jin, Hak-Sung Kim, Yosun Hwang, Ja Hun Kwak, Je-Geun Park, Chae-Ho Shin, Jungbae Kim and Taeghwan Hyeon, “Preparation of a Magnetically Switchable Bioelectrocatalytic System Employing Cross-Linked Enzyme Aggregates in Magnetic Mesocellular Carbon Foam”, Angew. Chem. Int. Ed., 44, 7427-7432 (2005).


• 14. Byoung Chan Kim, Sujith Nair, Jungbae Kim, Ja Hun Kwak, Jay W. Grate, Seong H. Kim and Man Bock Gu, “Preparation of Biocatalytic Nanofibres with High Activity and Stability via Enzyme Aggregate Coating on Polymer Nanofibres”, Nanotechnology, 16, S382-S388 (2005).


• 15. Jungbae Kim, Jinwoo Lee, Hyon Bin Na, Byoung Chan Kim, Jong Kyu Youn, Ja Hun Kwak, Karam Moon, Eunwoong Lee, Jaeyun Kim, Jongnam Park, Alice Dohnalkova, Hyun Gyu Park, Man Bock Gu, Ho Nam Chang, Jay W. Grate and Taeghwan Hyeon, “A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica”, Small, 1, 1203-1207 (2005).


• 16. Dohoon Lee, Jinwoo Lee, Jungbae Kim, Jaeyun Kim, Hyon Bin Na, Ja Hun Kwak, Alice Dohnalkova, Jay W. Grate, Taeghwan Hyeon and Hak-Sung Kim, “Simple Fabrication of a Highly Sensitive and Fast Glucose Biosensor Using Enzymes Immobilized in Mesocellular Carbon Foam”, Adv. Mater., 17, 2828-2833 (2005).


• 17. Jaeyun Kim, Ji Eun Lee, Jinwoo Lee, Jung Ho Yu, Byoung Chan Kim, Kwangjin An, Yosun Hwang, Chae-Ho Shin, Je-Geun Park, Jungbae Kim and Taeghwan Hyeon, “Magnetic Fluorescent Delivery Vehicle using Uniform Mesoporous Silica Spheres Embedded with Monodisperse Magnetic and Semiconductor Nanocrystals”, J. Am. Chem. Soc., 128, 688-689 (2006).


• 18. Jungbae Kim, Jay W. Grate and Ping Wang, “Nanostructures for Enzyme Stabilization”, Chem. Eng. Sci., 61, 1017-1026 (2006).


• 19. Jungbae Kim, Hongfei Jia, and Ping Wang, “Challenges in Biocatalysis for Enzyme-based Biofuel Cells”, Biotechnol. Adv., 24 (3), 296-308 (2006).


• 20. Jungbae Kim, Hongfei Jia, Chang-won Lee, Seung-wook Chung, Ja Hun Kwak, Yongsoon Shin, Alice Dohnalkova, Byung-Gee Kim, Ping Wang and Jay W. Grate, “Single Enzyme Nanoparticles in Nanoporous Silica: A Hierarchical Approach to Enzyme Stabilization and Immobilization”, Enzyme. Microb. Tech., 39, 474-480 (2006).


• 21. Yeon Seok Kim, Byoung Chan Kim, Jin Hyung Lee, Jungbae Kim and Man Bock Gu, “Specific Detection of DNA using Quantum Dots and Magnetic Beads for Large Volume Samples”, Biotechnol. Bioproc. E., 11, 449-454 (2006).


• 22. Michael B. Fischback, Jong Kyu Youn, Xueyan Zhao, Ping Wang, Hyun Gyu Park, Ho Nam Chang, Jungbae Kim and Su Ha, “Miniature Biofuel Cells with Improved Stability under Continuous Operation”, Electroanal., 18, 2016-2022 (2006).


• 23. Eunkeu Oh, Dohoon Lee, Young-Pil Kim, Seung Youp Cha, Doo-Beyong Oh, Hyun Ah Kang, Jungbae Kim and Hak-Sung Kim, “Nanoparticle-Based Energy Transfer for Rapid and Simple Detection of Protein Glycosylation”, Angew. Chem. Int. Ed., 45, 7959-7963 (2006).


• 25. Sujith Nair, Jungbae Kim, Brad Crawford and Seong H. Kim, “Improving Biocatalytic Activity of Enzyme-Loaded Nanofibers by Dispersing Entangled Nanofiber Structure”, Biomacromolecules, 8, 1266-1270 (2007).


• 26. Jin Hyung Lee, Ee Taek Hwang, Byoung Chan Kim, Sun-Mi Lee, Byoung-In Sang, Yong Soo Choi, Jungbae Kim and Man Bock Gu, “Stable and Continuous Long-term Enzymatic Reaction using an Enzyme Nanofiber Composite”, Appl. Microbiol. Biot., 75 (6), 1301-1307 (2007).


• 27. Jinwoo Lee, Youjin Lee, Jong Kyu Youn, Hyon Bin Na, Taekyung Yu, Hwan Kim, Sang-Mok Lee, Yoon-Mo Koo, Ja Hun Kwak, Hyun Gyu Park, Ho Nam Chang, Misun Hwang, Je-Geun Park, Jungbae Kim and Taeghwan Hyeon, “Simple Synthesis of Functionalized Superparamagnetic Magnetite/Silica Core/Shell Nanoparticles and their Application as Magnetically Separable High-Performance Biocatalysts”, Small, 4(1), 143-152 (2008).


• 28. Moon Il Kim, Jungbae Kim, Jinwoo Lee, Sujeong Shin, Hyon Bin Na, Taeghwan Hyeon, Hyun Gyu Park and Ho Nam Chang, “1-Dimensional Crosslinked Enzyme Aggregates in SBA-15: Superior Catalytic Behavior to Conventional Enzyme Immobilization”, Micropor. Mesopor. Mat., 111(1-3), 18-23 (2008).


• 29. Gowoun Lee, Hongil Joo, Jungbae Kim and Jung-Heon Lee, “Development of Magnetically Separable Immobilized Lipase by Using Cellulose Derivatives and Their Application in Enantioselective Esterification of Ibuprofen”, J. Microbiol. Biotechnol., 18(2), 465-471 (2008).


• 30. Gowoun Lee, Jungbae Kim and Jung-heon Lee, “Development of Magnetically Separable Polyaniline Nanofibers for Enzyme Immobilization and Recovery”, Enzyme Microb. Technol., 42(6), 466-472 (2008).


• 31. Jungbae Kim, Jay W. Grate and Ping Wang, “Nanobiocatalysis and Its Potential Applications”, Trends in Biotechnol., 26(11), 639-646 (2008).


• 32. Byoung Chan Kim, Daniel Lopez-Ferrer, Sang-Mok Lee, Hye-Kyung Ahn, Sujith Nair, Seong H. Kim, Beom Soo Kim, Konstantinos Petritis, David G. Camp, Jay W. Grate, Richard D. Smith, Yoon-Mo Koo, Man Bock Gu and Jungbae Kim, “Highly Stable Trypsin-Aggregate Coatings on Polymer Nanofibers for Repeated Protein Digestion”, Proteomics, 9, 1893-1900 (2009) ( coverimagearticle ).


• 33. Yunxian Piao, Dohoon Lee, Jungbae Kim, Jaeyun Kim, Taeghwan Hyeon and Hak-Sung Kim, “High Performance Immunoassay Using Immobilized Enzyme in Nanoporous Carbon”, Analyst, 134, 926-932 (2009).


• 34. Jin Young Lee, Hyun Yong Shin, Jong Ho Lee, Yoon Seok Song, Seong Woo Kang, Chulhwan Park, Jung Bae Kim and Seung Wook Kim, “A Novel Enzyme-Immobilization Method for a Biofuel Cell”, J. Mol Cat. B: Enzym., 59, 274-278 (2009).


• 35. Hye-Jin Lee, Byoung Chan Kim, Kyung-Woo Kim, Young Keun Kim, Jungbae Kim and Min-Kyu Oh, “A Sensitive Method to Detect Escherichia Coli Based on Immunomagnetic Separation and Real-Time PCR Amplification of Aptamers”, Biosens. Bioelectron., 24, 3550-3555 (2009).


• 36. Kyung-Min Yeon, Chung-Hak Lee and Jungbae Kim, "Magnetic Enzyme Carrier for Effective Biofouling Control in the Membrane Bioreactor Based on Enzymatic Quorum Quenching ", Environ. Sci. Technol., 43, 7403-7409 (2009).


• 37. Jinwoo Lee, Hyon Bin Na, Byoung Chan Kim, Jin Hyung Lee, Byoungsoo Lee, Ja Hun Kwak, Yosun Hwang, Je-Geun Park, Man Bock Gu, Jaeyun Kim, Jin Joo, Chae-Ho Shin, Jay W. Grate, Taeghwan Hyeon, and Jungbae Kim, “Magnetically-Separable and Highly-Stable Enzyme System Based on Crosslinked Enzyme Aggregates Shipped in Magnetite-Coated Hierarchically-Ordered Mesocellular Mesoporous Silica”, J. Mater. Chem., 19, 7864-7870 (2009).


• 38. Yunxian Piao, Dohoon Lee, Jinwoo Lee, Taeghwan Hyeon, Jungbae Kim and Hak-Sung Kim, “Multiplexed Immunoassay Using the Stabilized Enzymes in Mesoporous Silica”, Biosens. Bioelectron., 25, 906-912 (2009).


• 39. Xueyan Zhao, Hongfei Jia, Jungbae Kim and Ping Wang, “Kinetic Limitations of a Bioelectrochemical Electrode Using Carbon Nanotube-Attached Glucose Oxidase for Biofuel Cells”, Biotechnol. Bioeng., 104, 1068-1074 (2009).


• 40. Sang-Mok Lee, Li Hua Jin, Jae Hyun Kim, Sung Ok Han, Hyon Bin Na, Taeghwan Hyeon, Yoon-Mo Koo, Jungbae Kim and Jung-Heon Lee, “β-Glucosidase Coating on Polymer Nanofibers for Improved Cellulosic Ethanol Production”, Bioprocess Biosyst. Eng., 33, 141-147 (2010).


• 41. Jungbae Kim, Byoung Chan Kim, Daniel Lopez-Ferrer, Konstantinos Petritis and Richard D. Smith, “Nanobiocatalysis for Protein Digestion in Proteomic Analysis”, Proteomics, 10, 687-699 (2010).


• 42. Seung-Hyun Jun, Mun Seock Chang, Byoung Chan Kim, Hyo Jin An, Daniel Lopez-Ferrer, Rui Zhao, Richard D. Smith, Sang-Won Lee and Jungbae Kim, "Trypsin Coatings on Electrospun and Alcohol-Dispersed Polymer Nanofibers for Trypsin Digestion Column", Anal. Chem., 82, 7828-7834 (2010).


• 43. Sang-Mok Lee, Sujith Nair, Hye-Kyung Ahn, Beom Soo Kim, Seung-Hyun Jun, Hyo Jin An, Erik Hsiao, Seong H. Kim, Yoon-Mo Koo and Jungbae Kim, "Property Control of Enzyme Coatings on Polymer Nanofibers by Varying the Conjugation Site Concentration", Enzyme Microb. Technol., 47, 216-221 (2010).


• 44. Ki Young Kwon, Jongkyu Youn, Jae Hyun Kim, Yongjin Park, Chulmin Jeon, Byoung Chan Kim, Yongchai Kwon, Xueyan Zhao, Ping Wang, Byoung In Sang, Jinwoo Lee, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Su Ha, Hee-Tae Jung and Jungbae Kim, "Nanoscale Enzyme Reactors in Mesoporous Carbon for Improved Performance and Lifetime of Biosensors and Biofuel Cells", Biosens. Bioelectron., 26, 655-660 (2010).


• 45. Ki Young Kwon, Seungbo Yang, Byung-Seon Kong, Jungbae Kim and Hee-Tae Jung, "High-Performance Biosensors Based on Enzyme Precipitate Coating in Gold Nanoparticle-Conjugated SWNT Network Films", Carbon, 48, 4504-4509 (2010).


• 46. Hye-Kyung Ahn, Byoung Chan Kim, Seung Hyun Jun, Mun Seock Chang, Dani Lopez-Ferrer, Richard D. Smith, Man Bock Gu, Sang-Won Lee, Beom Soo Kim and Jungbae Kim, "Robust Trypsin Coating on Electrospun Polymer Nanofibers in Rigorous Conditions and Its Uses for Protein Digestion", Biotechnol. Bioeng., 107, 917-923 (2010).


• 47. Byoung Chan Kim, Xueyan Zhao, Hye-Kyung Ahn, Jae Hyun Kim, Hye-Jin Lee, Kyung W. Kim, Sujith Nair, Erik Hsiao, Hongfei Jia, Min-Kyu Oh, Byoung I. Sang, Beom-Soo Kim, Seong H. Kim, Yongchai Kwon, Su Ha, Man Bock Gu, Ping Wang and Jungbae Kim, "Highly Stable Enzyme Precipitate Coatings and Their Electrochemical Applications", Biosens. Bioelectron., 26, 1980-1986, (2011).


• 48. Byoungsoo Lee, Daniel Lopez-Ferrer, Byoung Chan Kim, Hyon Bin Na, Yong Il Park, Karl K. Weitz, Marvin G. Warner, Taeghwan Hyeon, Sang-Won Lee, Richard D. Smith and Jungbae Kim, "Rapid and Efficient Protein Digestion Using Trypsin Coated Magnetic Nanoparticles under Pressure Cycles", Proteomics, 11, 309-318 (2011) ( coverimagearticle ).


• 49. Yunxian Piao, Zongwen Jin, Dohoon Lee, Hye.-Jin Lee, Hyon-Bin Na, Taeghwan Hyeon, Min-Kyu Oh, Jungbae Kim and Hak-Sung Kim, "Sensitive and High-Fidelity Electrochemical Immunoassay Using Carbon Nanotubes Coated with Enzymes and Magnetic Nanoparticles", Biosens. Bioelectron., 26, 3192-3199 (2011).


• 50. Ee Taek Hwang, Rameshwar Tatavarty, Hyun Lee, Jungbae Kim and Man Bock Gu, “Shape Reformable Polymeric Nanofibers Entrapped with QDs as a Scaffold for Enzyme Stabilization”, J. Mater. Chem., 21, 5165-5520 (2011) ( coverimagearticle ).


• 51. Kyung-Woo Kim, Byoung Chan Kim, Hye-Jin Lee, Jungbae Kim and Min-Kyu Oh, "Enzyme Logic Gates Based on Enzyme-Coated Carbon Nanotubes", Electroanal., 23, 980-986 (2011).


• 52. Hyeongseok Kim, Inseon Lee, Yongchai Kwon, Byoung Chan Kim, Su Ha, Jung-heon Lee and Jungbae Kim, “Immobilization of Glucose Oxidase into Polyaniline Nanofiber Matrix for Biofuel Cell Applications”, Biosens. Bioelectron., 26, 3908-3913 (2011).


• 53. Hyo Jin An, Hye-Jin Lee, Seung-Hyun Jun, Sang Youn Hwang, Byoung Chan Kim, Kwanghee Kim, Kyung-Mi Lee, Min-Kyu Oh and Jungbae Kim, “Enzyme Precipitate Coatings of Lipase on Polymer Nanofibers”, Bioprocess Biosyst. Eng., 34, 841-847 (2011).


• 54. Byoung Chan Kim, Jinwoo Lee, Wooyong Um, Jaeyun Kim, Jin Joo, Jin Hyung Lee, Ja Hun Kwak, Jae Hyun Kim, Changha Lee, R. Shane Addleman, Taeghwan Hyeon, Man Bock Gu and Jungbae Kim, "Magnetic Mesoporous Materials for Removal of Environmental Wastes", J. Hazard. Mater., 192, 1140-1147 (2011).


• 55. Ho Nam Chang, Nag-Jong Kim, Jongwon Kang, Chang Moon Jeong, Jin-dal-rae Choi, Qiang Fei, Byoung Jin Kim, Sunhoon Kwon, Sang Yup Lee and Jungbae Kim, “Multi-Stage High Cell Continuous Fermentation for High Productivity And Titer”, Bioprocess Biosyst. Eng., 34, 419-431 (2011).


• 56. Hong Kyung Choi, Jeong Ho Chang, Il Hwan Ko, Jin Hyung Lee, Bong Yong Jeong, Jong Hee Kim and Jungbae Kim, “Electrostatic Interaction Effect for Human DNA Separation with Functionalized Mesoporous Silicas”, J. Solid State Chem., 184, 805-810 (2011).


• 57. Mike Fischback, Ki Young Kwon, Inseon Lee, Su Jeong Shin, Hyun Gyu Park, Byoung Chan Kim, Yongchai Kwon, Hee-Tae Jung, Jungbae Kim and Su Ha, "Enzyme Precipitate Coatings of Glucose Oxidase onto Carbon Paper for Biofuel Cell Applications", Biotechnol. Bioeng., 109, 318-324 (2012) (spotlightarticle).


• 58. Seung-Hyun Jun, Jinwoo Lee, Byoung Chan Kim, Jin Joo, Hyunmin Park, Jong Ho Lee, Sang-Mok Lee, Dohoon Lee, Sangyong Kim, Yoon-Mo Koo, Chae Ho Shin and Seung Wook Kim, Taeghwan Hyeon, and Jungbae Kim, “Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-Like Silica for Biodiesel Production”, Chem. Mater., 24, 924-929 (2012).


• 59. Hye-Jin Lee, Byoung Chan Kim, Min-Kyu Oh and Jungbae Kim, “A Sensitive and Reliable Detection of Thrombin via Enzyme-Precipitate-Coating-Linked Aptamer Assay”, Chem. Commun., 48, 5971-5973 (2012) ( coverimagearticle ).


• 60. Ee Taek Hwang, Byoungsoo Lee, Meiling Zhang, Seung-Hyun Jun, Jongmin Shim, Jinwoo Lee, Jungbae Kim and Man Bock Gu, “Immobilization and Stabilization of Subtilisin Carlsberg in Magnetically-Separable Mesoporous Silica for the Transesterification in an Organic Solvent”, Green Chem., 14, 1884-1887 (2012).


• 61. Kwanghee Kim, Hyo Jin An, Seung-Hyun Jun, Tae-Jin Kim, Gayoung Park, Hyon Bin Na, Yong Il Park, Taeghwan Hyeon, Cassian Yee, Jeffrey A Bluestone, Jungbae Kim and Kyung-Mi Lee, “Single Step Isolation and Activation of Primary CD3+ T Lymphocytes using Alcohol-Dispersed Electrospun Magnetic Nanofibers”, Nano Lett., 12, 4018-4024 (2012).


• 62. Kyoungseon Min, Jungbae Kim, Kyungmoon Park and Young Je Yoo, “Enzyme Immobilization on Carbon Nanomaterials: Loading Density Investigation and Zeta Potential Analysis”, J. Mol. Catal. B: Enzym., 83, 87-93 (2012).


• 63. Heechan Kim, Wooyul Kim, Yuri Mackeyev, Gi-Seon Lee, Hee-Joon Kim, Takashi Tachikawa, Seokwon Hong, Sanghyup Lee, Jungbae Kim, Lon J. Wilson, Tetsuro Majima, Pedro J. J. Alvarez, Wonyong Choi and and Jaesang Lee, “Selective Oxidative Degradation of Organic Pollutants by Singlet Oxygen-Mediated Photosensitization: Tin Porphyrin versus C-60 Aminofullerene Systems”, Environ. Sci. Technol., 46, 9606-9613 (2012).


• 64. Seung-Hyun Jun, Kwanghee Kim, Hyo Jin An, Byoung Chan Kim, Chung Hee Sonn, Kyung-Mi Lee and Jungbae Kim, “Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4+ T Lymphocytes”, Adv. Funct. Mater., 22, 4448-4455 (2012) (coverimagearticle).


• 65. Sadhana Rayalu, Renu Yadav, Snehal Wanjari, Chandan Prabhu, Srinivas Chakravarthy Mushnoori, Nitin Labhsetwar, T. Satyanarayanan, Swati Kotwal, S. R. Wate, Sung-Gil Hong and Jungbae Kim, “Nanobiocatalysts for Carbon Capture, Sequestration and Valorisation”, Top. Catal., 55, 1217-1230 (2012).


• 66. Su Ha, Youngho Wee and Jungbae Kim, “Nanobiocatalysis for Enzymatic Biofuel Cells”, Top. Catal., 55, 1181-1200 (2012).


• 67. Hee Uk Lee, Hah Young Yoo, Tseveg Lkhagvasuren, Yoon Seok Song, Chulhwan Park, Jungbae Kim and Seung Wook Kim, “Enzymatic Fuel Cells based on Electrodeposited Graphite Oxide/Cobalt Hydroxide/Chitosan Composite-Enzyme electrode”, Biosens. Bioelectron., 42, 342-348 (2013).


• 68. Young Hyun Kim, Inseon Lee, Sung Hee Choi, Ok Kyung Lee, Jongmin Sim, Jinwoo Lee, Jungbae Kim and Eun Yeol Lee, “Enhanced Stability and Reusability of Marine Epoxide Hydrolase Shipped-in-a-bottle into Magnetically-Separable Mesoporous Silica”, J. Mol. Catal. B: Enzym., 89, 48-51 (2013).


• 69. Young Hyun Kim, Inseon Lee, Sung Hee Choi, Ok Kyung Lee, Jungbae Kim and Eun Yeol Lee, “Nanoimmobilization of Marine Epoxide Hydrolase of Mugil cephalus for Repetitive Enantioselective Resolution of Racemic Styrene Oxide in Aqueous Buffer”, J. Nanosci. Nanotechnol., 13, 2266-2271 (2013).


• 70. Hyeon Jeong Kim, Siseon Lee, Jungbae Kim, Robert J. Mitchell and Jin Hyung Lee, “Environmentally Friendly Pretreatment of Plant Biomass by Planetary and Attrition Milling”, Bioresource Technol., 144, 50-56 (2013).


• 71. Xueyan Zhao, Xin Lu, William Tai Yin Tze, Jungbae Kim and Ping Wang, “Cellulosic Carbon Fibers with Branching Carbon Nanotubes for Enhanced Electrochemical Activities for Bioprocessing Applications”, ACS Appl. Mater. Interfaces, 5, 8853-8856 (2013).


• 72. Sae Rom Choi, Dong-Jin Jang, Sanghyun Kim, Sunhyung An, Jinwoo Lee, Euichaul Oh and Jungbae Kim, “Polymer-Coated Spherical Mesoporous Silica for pH-Controlled Delivery of Insulin”, J. Mater. Chem. B, 2, 616-619 (2014).


• 73. Sung-Gil Hong, Han Sol Kim and Jungbae Kim, “Highly Stabilized Lipase in Polyaniline Nanofibers for Surfactant-Mediated Esterification of Ibuprofen”, Langmuir, 30, 911-915 (2014).


• 74. Ji Young Eum, Sang Youn Hwang, Youngjun Ju, Jong Min Shim, Yunxian Piao, Jinwoo Lee, Hak-Sung Kim and Jungbae Kim, “A Highly Sensitive Immunoassay using Antibodies-Conjugated Spherical Mesoporous Silica with Immobilized Enzymes”, Chem. Commun., 50, 3546-3548 (2014) ( coverimagearticle ).


• 75. Byoungsoo Lee, Kyung-Min Yeon, Jongmin Shim, Sang-Ryoung Kim, Chung-Hak Lee, Jinwoo Lee and Jungbae Kim, “Effective Antifouling Using Quorum-Quenching Acylase Stabilized in Magnetically-Separable Mesoporous Silica”, Biomacromolecules, 15, 1153-1159 (2014).


• 76. Shunxiang Xia, Benjamin Frigo-Vaz, Xueyan Zhao, Jungbae Kim and Ping Wang, “Biocatalytic Carbon Capture via Reversible Reaction Cycle Catalyzed by Isocitrate Dehydrogenase”, Biochem. Biophys. Res. Commun., 452, 147-150 (2014).


• 77. Ryang Eun Kim, Sung-Gil Hong, Su Ha and Jungbae Kim, “Enzyme Adsorption, Precipitation and Crosslinking of Glucose Oxidase and Laccase on Polyaniline Nanofibers for Highly Stable Enzymatic Biofuel Cells”, Enzyme Microb. Technol., 66, 35-41 (2014).


• 78. Ki Young Kwon, Jae Hyun Kim, Jongkyu Youn, Chulmin Jeon, Jinwoo Lee, Taeghwan Hyeon, Hyun Gyu Park, Ho Nam Chang, Yongchai Kwon, Su Ha, Hee-Tae Jung and Jungbae Kim, “Electrochemical Activity Studies of Glucose Oxidase (GOx)-based and Pyranose Oxidase (POx)-based Electrodes in Mesoporous Carbon: Toward Biosensor and Biofuel Cell Applications”, Electroanalysis., 26, 2075-2079 (2014).


• 79. Shunxiang Xia, Ping Wang and Jungbae Kim, “pH Sensitivity of Conversion of Carbon Dioxide Catalyzed by Isocitrate Dehydrogenase”, Greenh. Gases, 5, 102-107 (2015).


• 80. Jae Hyun Kim, Sun-Ae Jun, Yongchai Kwon, Su Ha, Byong-In Sang and Jungbae Kim, “Enhanced Electrochemical Sensitivity of Enzyme Precipitate Coating (EPC)-based Glucose Oxidase Biosensors with Increased Free CNT Loadings”, Bioelectrochemistry, 101, 114-119 (2015).


• 81. Shunxiang Xia, Xueyan Zhao, Benjamin Frigo-Vaz, Wenyun Zheng, Jungbae Kim and Ping Wang, “Cascade Enzymatic Reactions for Efficient Carbon Sequestration”, Bioresour. Technol., 182, 368-372 (2015).


• 82. Sung-Gil Hong, Hancheol Jeon, Han Sol Kim, Seung-Hyun Jun, EonSon Jin and Jungbae Kim, “One-pot Enzymatic Conversion of Carbon Dioxide and Utilization for Improved Microbial Growth”, Environ. Sci. Technol., 49, 4466-4472 (2015).


• 83. Kie Moon Woo, Inseon Lee, Sung-Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh and Jungbae Kim, “Crosslinked Chitosan Coating on Magnetic Mesoporous Silica with Pre-adsorbed Carbonic Anhydrase for Carbon Dioxide Conversion”, Chem. Eng. J., 276, 232-239 (2015).


• 84. Gudi Satheesh Kumar, Youngho Wee, Inseon Lee, Ho Jin Sun, Xueyan Zhao, Shunxiang Xia, Seongbeen Kim, Jinwoo Lee, Ping Wang and Jungbae Kim, “Stabilized Glycerol Dehydrogenase for the Conversion of Glycerol to Dihydroxyacetone”, Chem. Eng. J., 276, 283-288 (2015) ( cover image article ).


• 85. Jinho Yoon, Yong-Ho Chung, Taek Lee, Jae Hyun Kim, Jungbae Kim and Jeong-Woo Choi, “A Biomemory Chip Composed of a Myoglobin/CNT Heterolayer Fabricated by the Protein-adsorption-precipitation-crosslinking (PAPC) Technique”, Colloids and Surfaces B: Biointerfaces, 136, 853-858 (2015).


• 86. Jae Hyun Kim, Sung-Gil Hong, Ho Jin Sun, Su Ha and Jungbae Kim, “Precipitated and Chemically-Crosslinked Laccase over Polyaniline Nanofiber for High Performance Phenol Sensing”, Chemosphere, 143, 142-147 (2016).


• 87. Byoungsoo Lee, Byoung Chan Kim, Mun Seock Chang, Han Sol Kim, Hyon Bin Na, Yong Il Park, Jinwoo Lee, Taeghwan Hyeon, Hookeun Lee, Sang-Won Lee and Jungbae Kim, “Efficient Protein Digestion using Highly-Stable and Reproducible Trypsin Coatings on Magnetic Nanofibers”, Chem. Eng. J. 288, 770-777 (2016).


• 88. Tsai Garcia-Perez, Sung-Gil Hong, Jungbae Kim and Su Ha, “EntrappingCross-linked Glucose Oxidase Aggregates within a Graphitized Mesoporous Carbon Network for Enzymatic Biofuel Cells”, Enzyme Microb. Technol. 90, 26-34 (2016).


• 89. Na Liu, Gang Liang, Xinwei Dong, Xiaoli Qi, Jungbae Kim and Yunxian Piao, “Stabilized Magnetic Enzyme Aggregates on Graphene Oxide for High Performance Phenol and Bisphenol A Removal”, Chem. Eng. J. 306, 1026-1034 (2016).


• 90. Sung-Gil Hong, Jae Hyun Kim, Ryang Eun Kim, Seok-Joon Kwon, Dae Woo Kim, Hee-Tae Jung, Jonathan S. Dordick and Jungbae Kim, “Immobilization of Glucose Oxidase on Graphene Oxide for Highly Sensitive Biosensors”, Biotechnol. Bioprocess Eng. 21, 573-579 (2016).


• 91. Jae Hyun Kim, Sung-Gil Hong, Youngho Wee, Shuozhen Hu, Yongchai Kwon, Su Ha and Jungbae Kim, “Enzyme Precipitate Coating of Pyranose Oxidase on Carbon Nanotubes and Their Electrochemical Applications”, Biosens. Bioelectron. 87, 365-372 (2017).


• 92. Byoung Chan Kim, Inseon Lee, Seok-Joon Kwon, Youngho Wee, Ki Young Kwon, Chulmin Jeon, Hyo Jin An, Hee-Tae Jung, Su Ha, Jonathan S. Dordick and Jungbae Kim, “Fabrication of Enzyme-based Coatings on Intact Multi-walled Carbon Nanotubes as Highly Effective Electrodes in Biofuel Cells”, Sci. Rep. 7, 40202 (2017).


• 93. Sung-Gil Hong, Byoung Chan Kim, Hyon Bin Na, Jinwoo Lee, Jongkyu Youn, Seung-Wook Chung, Chang-Won Lee, Byoungsoo Lee, Han Sol Kim, Erik Hsiao, Seong H. Kim, Byung-Gee Kim, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Jonathan S. Dordick, Jay W. Grate and Jungbae Kim “Single Enzyme Nanoparticles Armored by a Thin Silicate Network: Single Enzyme Caged Nanoparticles”, Chem. Eng. J., 322, 510-515 (2017).


• 94. Xia Wu, Seok-Joon Kwon, Jungbae Kim, Ravi S. Kane and Jonathan S. Dordick, “Biocatalytic Nanocomposites for Combating Bacterial Pathogens”, Annu. Rev. Chem. Biomol. Eng., 8, 87-113 (2017).


• 95. Seok-Joon Kwon, Domyoung Kim, Inseon Lee, Jungbae Kim and Jonathan S. Dordick, “In Vitro Gene Expression-Coupled Bacterial Cell Chip for Screening Species-Specific Antimicrobial Enzymes”, Biotechnol. Bioeng., 114, 1648-1657 (2017).


• 96. Hwa Heon Je, Sora Noh, Sung-Gil Hong, Youngjun Ju, Jungbae Kim and Dong Soo Hwang, “Cellulose Nanofibers for Magnetically-Separable and Highly Loaded Enzyme Immobilization”, Chem. Eng. J., 323, 425-433 (2017).


• 97. Jeongjoon Lee, Inseon Lee, Jahyun Nam, Dong Soo Hwang, KyungMin Yeon and Jungbae Kim, “Immobilization and Stabilization of Acylase on Carboxylated Polyaniline Nanofibers for Highly Effective Antifouling Application via Quorum Quenching”, ACS Appl. Mater. Interfaces., 9, 15424-15432 (2017).


• 98. Junghyo Yoon, Hee-Sook Yoon, Yoojin Shin, Sanghyun Kim, Youngjun Ju, Jungbae Kim and Seok Chung, “Ethanol-dispersed and Antibody-conjugated Polymer Nanofibers for the Selective Capture and 3-dimensional Culture of EpCAM-positive Cells”, Nanomed.-Nanotechnol. Biol. Med., 13, 1617-1625 (2017).


• 99. Hyeonil Kim, Han Sol Kim, Dabin Lee, Dongwon Shin, Daeho Shin, Jeongkwon Kim and Jungbae Kim, “Microwave-assisted Protein Digestion in a Plate Well for Facile Sampling and Rapid Digestion”, Anal. Chem., 89, 10655-10660 (2017).


• 100. Tae Hee Kim, Inseon Lee, Kyung-Min Yeon and Jungbae Kim, “Biocatalytic Membrane with Acylase Stabilized on Intact Carbon Nanotubes for Effective Antifouling via Quorum Quenching”, J. Membr. Sci., 554, 357-365 (2018).


• 101. Domyoung Kim, Seok-Joon Kwon, Jessica Sauve, Inseon Lee, Jahyun Nam, Jungbae Kim and Jonathan S. Dordick, “Selective Killing of Pathogenic Bacteria by Antimicrobial Silver Nanoparticle - Cell Wall Binding Domain (CBD) Conjugates”, ACS Appl. Mater. Interfaces., 10, 13317-13324 (2018).


• 102. Han Sol Kim, Sung-Gil Hong, Kie Moon Woo, Vanesa Teijeiro Seijas, Seongbeen Kim, Jinwoo Lee and Jungbae Kim, “Precipitation-Based Nanoscale Enzyme Reactor with Improved Loading, Stability and Mass Transfer for Enzymatic CO2ConversionandUtilization”, ACS Catal., 8, 6526-6536 (2018).


• 103. Seok-Joon Kwon, Domyoung Kim, Inseon Lee, Jahyun Nam, Jungbae Kim and Jonathan S. Dordick, “Sensitive Multiplex Detection of Whole Bacteria Using Self-Assembled Cell Binding Domain Complexes”, Anal. Chim. Acta. (accepted).

Nanobiocatalysis

Enzymes are eco-friendly biocatalysts that catalyze chemical reactions of living organisms. Because of their unique substrate specificity and very fast reaction rate, they attract attentions of many researchers and are estimated as potentially useful in the catalytic reaction fields.

Furthermore, they attain biological activity at moderate operational conditions and produce little byproduct. Due to these reasons, enzymes are highly valuable in the industrial fields and already they are currently used for wide range of industrial sectors.

Enzyme have vital role in biotechnological industries such as, pharmaceutics, fine chemistry, food industry, detergent industry, biosensors, anti-fouling, bioconversion, biodiesel production, trypsin digestion in proteomics, polymerase chain reaction (PCR), biofuel cells, and so forth.

In spite of their merits in industrial fields, enzymes are restricted their usefulness due to the denaturation of enzyme structure and short lifespan of enzymes caused by their instability.

There have been a lot of attempts to solve instability of enzymes by using various technologies such as enzyme immobilization, chemical mutation of enzymes, protein engineering, gene manipulation,and solvent engineering.

Those stabilization approaches have seems to achieve improved stability when compared to free enzyme, but it was not sufficient level to apply stabilized enzymes to real industrial applications.

Recent remarkable developments offer the solutions of enzyme stabilization issues.

Nanomaterials that include nanoporous materials, nanofibers, nanoparticles, nanotubes have ideal properties to enzyme immobilization and they provide large surface to volume ratio that makes much more enzyme loading compared to convention carriers used in enzyme immobilization. Recently published research results that represent the synergetic interactions of enzyme immobilization with nanomaterials have opened a new prospect in the field of “Nanobiocatalysis”.

Nanobiocatalysis laboratory has three original base platforms, enzyme coating, nanoscale enzyme reactor, and single enzyme nanoparticles which are the results of the convergence of nanotechnologies and enzyme technologies.

Based on these platforms, nanobiocatalysis laboratory looking for breakthorough in enzyme stabilization and immobilization experiments in nanobiocatalysis fields.

Enzyme stabilization and immobilization technologies by using nanobiocatalysis approaches show remarkable stabilization results that cannot be achieved by conventional stabilization technologies.

Also, it is possible to apply such nanobiocatalytic platforms to various industrial or research fields because nanobiocatalytic platforms are not limited only a few enzymes but can be widely used for most of the industrially significant enzymes.

In the light of above information, it is prospected the possibility to solve the instability of free enzymes that is pointed out as the serious problem. Through stabilization of enzyme, the potential usefulness of enzymes is expected to dramatically increase.